The study examines the relationship between the accessibility of municipal infrastructure and the potential for brownfield regeneration in Czechia. The aim is to analyse how the level of infrastructure differs across regions, how these differences influence the business attractiveness of territories, and what implications arise for public policies supporting development. The Accessibility of Municipal Infrastructure (AMI) index was constructed from CzechInvest’s National Brownfields Database to quantify regional disparities in the connection of brownfield sites to five categories of utility networks: electricity, potable water, sewage, gas, and telecommunications. The results show pronounced differences between industrially developed and peripheral regions, confirming that infrastructural accessibility strongly determines both redevelopment feasibility and regional competitiveness. Regions such as Pardubice, South Moravia and Pilsen display the highest AMI values, while South Bohemian and Liberec regions remain structurally disadvantaged. The findings highlight that infrastructure represents not only a technical but also an institutional and strategic determinant of territorial development. Improving infrastructural readiness in lagging regions should therefore be a priority for Czech regional policy, as it directly enhances investment attractiveness, innovation potential, and the efficiency of public support instruments. The AMI framework provides a replicable tool for evaluating regional disparities and designing evidence-based development strategies.